O-3: In-Situ Preservation of Caprine CaudaEpididymal Spermatozoa at -10 Degree Centigrade
نویسندگان
چکیده مقاله:
Background: In-situ preservation of cauda epididymal spermatozoa at -10 degree centigrade with electrolyte free media for obtaining maximum functional gametes than preservation at 5 degree centigrade. Materials and Methods: Electrolyte free media prepared with soybean lecithin-glycerol, Coenzyme Q10 - glycerol and soybean lecithin - Coenzyme Q10- glycerol were inoculated separately into ligated cauda epididymides, equilibrated 2 h at 5 degree centigrade,centigrade. Spermatozoan characters were evaluated 7 and 21 days after thawing at 38.5 0C in a water bath for 5 min. Results: Spermatozoan characters were diminished gradually and significantly (p
منابع مشابه
O-10: PLAP Marker in Testicular Biopsy ofIranian Men by Immunohistochemistry Methodfor Early Diagnosis of Testicular Carcinomain Situ
Background: Testicular Carcinoma in situ (CIS), also known as intratubular germ cell neoplasia (ITGCN), is a pre-invasive precursor of testicular germ cell tumours (TCGTs), i.e seminomas and non-seminomas -the commonest cancer type of male adolescents and young adults- which can be diagnosed using a surgical biopsy. Infertility is one of the condition known to predispose to TGCT, but based on s...
متن کاملP-119: In Vitro Assessment of Sucrose for Preservation of Buffalo Epididymal Spermatozoa
Background: Epididymal spermatozoa have been used for production of offspring from genetically important animals after they are dead. To attain optimal fertility with epididymal spermatozoa, they should be preserved in a suitable medium in 37°C. Preservation media provides requirement for spermatozoa and affects its motility, viability, and fertility. Sucrose is one of the important components ...
متن کاملRealization of Degree 10 Minimum Spanning Trees in 3-Space
We show that any tree whose maximum degree is at most 10 can be drawn in 3-space such that it is the minimum spanning tree of its vertices.
متن کاملKinetics of water loss from cells at subzero centigrade temperatures.
The cells of which living things are com posed and the single-celled microorganisms generally contain very high fractions of water. The high fraction of water in cells introduces a problem when the cells are cooled below 0°C; that is, one has to find out what happens to the water content of the cells when they are cooled to tempera tures at which water freezes. There are many factors involved...
متن کاملGranulosa Cell Apoptosis in Situ in Caprine Ovary
Apoptotic changes in membrana granulosa of ovaries in goat (Capra hircus) have been studied in situ using light and scanning electron microscopy. Histologically, the degenerating granulosa cells were characterized by condensed cytoplasm, pyknotic nuclei and hazy cytosol. Most of the cytoplasmic components were condensed and were seen clumping against the pyknotic nucleus. The nucleus of degener...
متن کاملCryopreservation of Spermatozoa: Recent Biotechnological Advancement in Gamete Preservation Technology
Cryopreservation of sperm has many applications in agriculture, biotechnology, and clinical medicine. The spermatozoon is a very specialized cell that loses the ability of biosynthesis, repair, growth and cell division during the final phase of spermatogenesis. Cryopreservation of sperm generally requires a reduction or arrest of the metabolism of cells, thereby prolonging their life. Semen sam...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ذخیره در منابع من قبلا به منابع من ذحیره شده{@ msg_add @}
عنوان ژورنال
دوره 4 شماره 2
صفحات 64- 64
تاریخ انتشار 2010-05-01
با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023